Single-cell transcriptomic profiling reveals aberrant CD4⁺ naive T cell differentiation driving immune activation in Behcet's uveitis.

ABSTRACT:

Background

Behçet's uveitis (BU) is a severe inflammatory ocular manifestation of Behçet's disease, characterized by dysregulated T cell responses. However, the precise mechanisms underlying T cell dysfunction and local immune activation in BU remain poorly understood.

Methods

We performed single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells (PBMCs) from BU patients and healthy controls (HCs). These data were integrated with publicly available single-cell transcriptomes of aqueous humor (AH) samples from BU patients. Key findings were validated using qPCR, flow cytometry, and functional assays.

Results

We observed a significant depletion of naive CD4⁺ T cells in BU, accompanied by their differentiation toward terminal effector states characteri

SUBMITTER: Zhang L 

PROVIDER: S-EPMC12777324 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature