Boric acid and quercetin supplementations alleviated paraquat-induced neurotoxic and irritation effects in human SH-SY5Y cells and in ovo models.

ABSTRACT:

Background

Paraquat (PQ) is a herbicide that causes neurotoxicity through oxidative stress, mitochondrial dysfunction and apoptosis. Boric acid (BA) and quercetin (Qe) are bioactive compounds with antioxidant and anti-inflammatory properties, but their combined effect against PQ-induced neuronal damage is still unexplored. This study aimed to investigate the individual and synergistic protective effects of BA and Qe in SH-SY5Y neuroblastoma cells and in ovo models.

Methods

Human SH-SY5Y neuroblastoma cells were exposed to PQ (400 µM) with or without BA (50 µM), Qe (50 µM) or their combination for 48 h. ROS production, mitochondrial membrane potential (MMP, ΔΨm) and intracellular calcium levels (Ca²⁺) were measured. Apoptotic (BAX, BCL2, Casp-3) and inflammatory (NFKB, VEGF,

SUBMITTER: Guner A 

PROVIDER: S-EPMC12777461 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature