Exploring RNA G-Quadruplex Stability in the Gas Phase: Insights from Native Mass Spectrometry.

ABSTRACT: Native mass spectrometry (MS) of ribonucleic acids (RNA) is a developing technique for the study of RNA structure and interactions that can provide important information on binding stoichiometry and binding motifs. However, the stabilities of key structural elements of RNA, including G-quadruplex structures, during and after desolvation are not well understood. Efforts to improve our understanding of RNA structures in the gas phase are complicated by the fact that the most common technique for transferring RNA from solution to the gas phase, electrospray ionization (ESI), typically produces ions with a relatively wide distribution of net charges. Here, we have studied two tetramolecular RNA G-quadruplexes with very different stabilities in solution by native MS. For both K⁺ and

SUBMITTER: Ploner A 

PROVIDER: S-EPMC12777508 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature