Whole-body distribution of three <i>Pseudomonas</i> phages characterized by a translational physiologically based pharmacokinetic model.

ABSTRACT: Bacteriophage (phage) therapy holds great promise for treating antimicrobial-resistant infections. However, the pharmacokinetics (PK) of phage have been difficult to characterize due to a lack of standardized protocols for phage purification, labeling, and in vivo quantification. Here, we present robust methods for ultrapure phage preparation, as well as non-destructive, highly stable attachment of radio-iodide to phage using a well-described Sulfo-SHPP linker. We purified and radiolabeled the phage strains, PAML-31-1, OMKO1, and Luz24, lytic to drug-resistant Pseudomonas aeruginosa, for biodistribution assay in normal young adult CD-1 mice injected via intravenous injection. Groups of five mice were euthanized, and tissues/organs were removed for weighing and scintillation w

SUBMITTER: Echterhof A 

PROVIDER: S-EPMC12777566 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature