Vendor-specific microbiomes influence oral cancer development and its response to <i>Streptococcus mitis</i> intervention in mice.

ABSTRACT:

Background

We previously demonstrated that Streptococcus mitis exhibits anticancer properties in vitro. Here, we sought to validate these findings in vivo. Because mice from different vendors harbor distinct microbiomes that can influence disease susceptibility and experimental outcomes, we also examined whether vendor-specific oral and gut microbiomes affect oral carcinogenesis and response to S. mitis intervention.

Materials and methods

Oral carcinogenesis was induced using 4-NQO in C57BL/6 mice from Jackson Laboratory and Taconic Biosciences (n = 32 per vendor). Mice were randomized to biweekly oral swabbing with S. mitis or vehicle for 28 weeks. Oral and fecal microbiomes were profiled at baseline and week 8. At week 32, tongues

SUBMITTER: El-Hadedy DE 

PROVIDER: S-EPMC12777837 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature