Exploring techniques for extraction of silver fir (<i>Abies alba</i>): phytochemical composition, antioxidant activity and cell viability.

ABSTRACT:

Context

Silver fir (Abies alba) contains polyphenols and lignans with antioxidant and therapeutic properties. Efficient extraction methods are essential to preserve these compounds and maximize bioactivity.

Objective

To compare extraction techniques and identify the optimal method for obtaining high-quality silver fir extracts with strong antioxidant activity and minimal cytotoxicity.

Materials & methods

Extracts from bark and branches were prepared using subcritical water extraction (SWE, 70-200 °C), supercritical CO₂ extraction, and high-pressure ethanol extraction. Extracts were analyzed for total polyphenol content (TPC), lignan concentration (HPLC), antioxidant activity (DPPH and ABTS assays), and volatile profile (GC-MS). Cytotoxicity and cell

SUBMITTER: Schoss K 

PROVIDER: S-EPMC12777867 | biostudies-literature | 2026 Dec

REPOSITORIES: biostudies-literature