Cross-Cell line transcriptome profiling reveals host-Virus interactions in monkeypox virus infection.

ABSTRACT: The monkeypox virus (MPXV), a re-emerging Orthopoxvirus, has triggered unprecedented global outbreaks, with over 130,000 confirmed cases reported by January 2025. Despite its escalating public health threat, the molecular mechanisms governing MPXV-host interactions remain inadequately characterized. In this study, we systematically explored the impact of MPXV Clade IIb infection on the host transcriptome across diverse cell lines, unveiling both conserved and cell-type-specific transcriptomic changes. Our analysis revealed significant disruptions in pathways associated with immune response, cell cycle regulation, metabolism, protein synthesis, and epigenetic modification. Additionally, we evaluated two widely used mRNA sequencing methodologies for MPXV transcriptome profiling, demon

SUBMITTER: Yi H 

PROVIDER: S-EPMC12777890 | biostudies-literature | 2026 Dec

REPOSITORIES: biostudies-literature