METTL1-Mediated N⁷-Methylguanosine tRNA Modification Alleviates Cardiac Ischemia/Reperfusion Injury by Modulating Mitochondrial Energy Metabolism.

ABSTRACT: Ischemic heart disease is one of the diseases with the highest morbidity and mortality in the world. The N⁷-methylguanosine (m⁷G) tRNA modifications are widely recognized as one of the most prevalent tRNA modifications. Nevertheless, there is still a lack of understanding regarding the roles and molecular mechanisms underlying the METTL1-mediated m⁷G tRNA modification in cardiac ischemia/reperfusion (I/R) injury. METTL1 and m⁷G tRNA modification were upregulated in mice with I/R injury hearts and the plasma of patients with acute myocardial infarction. Thus, we constructed METTL1 knockout mice and found that silencing METTL1 alleviates I/R. Mechanistically, tRNA sequencing, MeRIP-m⁷G-tRNA sequencing, and Ribosome profiling sequencing w

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12778400 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature