The Cholinergic Pathway and MitoK_ATP Induce UCP4 Expression Involved in Neuroprotection of FN Stimulation in Rats.

ABSTRACT:

Background

Electrical stimulation of the cerebellar fastigial nucleus (FN) reduces the infarct size induced by middle cerebral artery occlusion in rats. FN stimulation confers long-lasting protection from brain injury; however, its underlying mechanism is not yet understood. We aimed to elucidate the mechanism by which FN stimulation exerts neuroprotection. We hypothesized that the neuroprotective effect of FN stimulation involves activation of cholinergic pathways, which increases reactive oxygen species (ROS) production by opening mitochondrial K⁺ _ATP channels, thus leading to an increase in UCP4 (uncoupling protein 4) expression and subsequent neuroprotection.

Methods

FN stimulation was performed for 1 hour in rats. The UCP4 protein and mRNA levels w

SUBMITTER: Fukushi Y 

PROVIDER: S-EPMC12778859 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature