Identification of stable internal reference genes for expression analysis in the liver and pancreas of diabetic mouse (Mus musculus L.) models under physiological, pathological and treatment conditions.

ABSTRACT: Accurate gene expression analysis via reverse transcription quantitative real-time PCR is vital for studying diabetes mellitus. Accurate and reliable normalization of RT-qPCR data, in accordance with the MIQE guidelines, demands a group of at least two stably expressed reference genes, especially in diabetic models where metabolic dysregulation often alters the profile of gene expression. To identify the best reference genes, this study systematically evaluates the stability of eleven candidate reference genes, including ACT, B2M, GAPDH, HPRT1, PPIA, RPL13A, RPLP0, TBP, UBC, YWHAZ, and 18SRNA, in liver, pancreas, and both types of tissues across four mouse groups of control, obesity, STZ-induced diabetic and treated STZ-diabetic groups. The identification of a robust group of reference gen

SUBMITTER: Nong VD 

PROVIDER: S-EPMC12779057 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature