Comparative Analysis of HEK293 Genomic Variability.

ABSTRACT: Human embryonic kidney cells HEK293 are widely used in biopharmaceutical manufacturing, with a recent surge particularly in recombinant adeno-associated virus production. Despite their industrial relevance, comprehensive data on their genomic background and stability remains limited. Here, we systematically analyze the genetic landscape of various HEK293 cell lines in response to cultivation conditions, clonal selection, genetic manipulation and over time in culture. Adherent HEK293 were adapted to suspension growth using different serum-free media. Whole genome sequences from these cell lines were analyzed together with previously published data from additional variants in common use. All data sets were aligned against the human reference genome, enabling the assessment of genome stabilit

SUBMITTER: Smesnik G 

PROVIDER: S-EPMC12779178 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature