Rapid tuberculosis diagnosis from respiratory or blood samples by a low cost, portable lab-in-tube assay.

ABSTRACT: Rapid portable assays are needed to improve diagnosis, treatment, and reduce transmission of tuberculosis (TB), but current tests are not suitable for patients in resource-limited settings with high TB burden. Here we report a low complexity, lab-in-tube system that is read by an integrated handheld device that detects Mycobacterium tuberculosis (Mtb) DNA in blood and respiratory samples from a variety of clinical settings. This microprocessor-controlled device uses an LCD user interface to control assay performance, automate assay analysis, and provide results in a simple readout. This point-of-care single-tube assay uses a DNA enrichment membrane and a low-cost cellulose disc containing lyophilized recombinase polymerase amplification and CRISPR-Cas12a reagents to attain si

SUBMITTER: Youngquist BM 

PROVIDER: S-EPMC12779336 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature