METTL3-mediated m6A modification of TACSTD2 mRNA inhibits papillary thyroid cancer progression by activating the TNF signaling and blocking epithelial-mesenchymal transition.

ABSTRACT: Papillary thyroid cancer (PTC) represents a prevalent endocrine neoplasm. Dysregulated tumor-associated calcium signal transducer 2 (TACSTD2) expression is functionally involved in the progression of multiple cancers. Nonetheless, the biological molecular mechanisms underlying TACSTD2 in PTC remain unclear. To verify the biological function of TACSTD2 in PTC, we conducted loss- and gain-of-function experiments, namely, cell proliferation (Cell Counting Kit-8), EdU incorporation, colony formation, and transwell migration/invasion assays, along with establishing a nude mouse xenograft tumor model. Our findings revealed that TACSTD2 knockout impeded PTC cell proliferation, migration, and invasion in vitro and reduced tumorigenicity in vivo. In contrast, TACSTD2 overexpression reversed these e

SUBMITTER: Man J 

PROVIDER: S-EPMC12780034 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature