The apoplastic protein OsCPIP8 confers dual protection by inhibiting pathogenic protease and activating rice immunity.

ABSTRACT: Cysteine proteases play key roles in plant immunity. In contrast, pathogen-secreted proteases contribute to destroying the immune defenses of plants and animals. However, the pathogenic mechanism remains enigmatic. Here, we find Xanthomonas oryzae pv. oryzicola (Xoc)-secreted cysteine proteinase XoCysP1 interacts with rice-secreted xylanase inhibitory protein OsXIP2, which enhances rice resistance to Xoc, and promotes OsXIP2 degradation in rice apoplast. As a counterattack, the rice-secreted cysteine protease inhibitory protein OsCPIP8 associates with XoCysP1 to block XoCysP1-mediated OsXIP2 degradation and confer rice resistance. Additionally, OsCPIP8 is recognized by the receptor-like kinases OsRLK8 and OsSERK2 to trigger plant immunity. In conclusion, OsCPIP8 not only protects defense p

SUBMITTER: Zhang J 

PROVIDER: S-EPMC12780185 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature