Loss of E3 ligase HvST1 function substantially increases distal crossover frequency.

ABSTRACT: This study characterizes the function of a grass-specific E3 ubiquitin ligase, HvST1, in regulating synapsis and crossover (CO) formation during meiosis in barley (Hordeum vulgare). In large-genome cereals, COs are predominantly restricted to distal chromosomal regions, limiting genetic recombination and breeding flexibility. We aimed to identify genetic components regulating CO frequency and distribution. A frameshift mutation in HvST1 was identified in the semi-sterile barley near-isogenic line BW233 through fine mapping of the des12.w locus. The causal role of this mutation was validated via CRISPR/Cas9 gene editing. HvST1 function was investigated using in vitro autoubiquitination and substrate ubiquitination assays, while meiotic progression was assessed using structured illumination

SUBMITTER: Orr JN 

PROVIDER: S-EPMC12780313 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature