A novel mechanism of auxin habituation: upregulation of auxin receptor TRANSPORT INHIBITOR RESPONSE 1 allows cell proliferation independent of external auxin.

ABSTRACT: Exogenously applied auxins are essential for establishing cell lines in tissue cultures and maintaining their proliferation. Cell lines may develop the ability to proliferate even in media lacking auxin, they may become auxin-habituated. This study investigated the mechanisms underlying this process. Here, we conducted comprehensive auxin metabolic profilings, pharmacological treatments and transcriptomic comparisons in two independently habituated tobacco cell lines, BY-2H and VBI-2, derived from cell lines of cultivars Bright Yellow (BY-2) and Virginia Bright Italia (VBI-0). Our results show that both habituated lines developed different mechanisms of auxin autonomy. In VBI-2, increased expression of MADS-domain transcription factor genes suggests epigenetically determined habituation. B

SUBMITTER: Jelinek P 

PROVIDER: S-EPMC12780323 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature