Bulb biopsies for diagnosing celiac disease in children: A prospective multicenter study using quantitative histomorphometry.

ABSTRACT:

Objectives

Duodenal bulb specimens are recommended for the diagnostic evaluation of celiac disease (CeD). However, it remains unclear if their quality and histomorphometry measures are comparable to samples obtained from the distal duodenum. We investigated this issue, as well as the diagnostic applicability of morphometry and advanced immunohistochemistry methods, in a prospective pediatric cohort using validated morphometric readouts.

Methods

One hundred ninety-four children with CeD autoantibodies underwent systematic sampling at diagnosis from bulb and distal duodenum. Morphometric evaluation included measurements of villous height (VH), crypt depth (CrD), and VH:CrD. Mucosal CD3+ and γδ+ intraepithelial lymphocytes (IEL) densities and transglutaminase-2-targeted immunog

SUBMITTER: Popp A 

PROVIDER: S-EPMC12780468 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature