LEAP2 triggers retromer-mediated membrane trafficking of MOSPD2 to promote chemotaxis in teleost monocytes/macrophages.

ABSTRACT: Liver-expressed antimicrobial peptide 2 (LEAP2) is a key regulator of innate immune defense in teleosts, yet the molecular basis of its chemotactic function remains largely unidentified. Boleophthalmus pectinirostris MOSPD2 ( BpMOSPD2) was previously identified as a candidate receptor for BpLEAP2 in monocytes/macrophages (MO/MΦ). In the present study, BpLEAP2 stimulation was found to trigger a retromer-dependent intracellular trafficking program essential for BpMOSPD2-mediated chemotaxis. Exposure to BpLEAP2 significantly enhanced BpMO/MΦ migration and promoted the accumulation of BpMOSPD2 at the plasma membrane. Subcellular fractionation and immunofluorescence analyses revealed that BpMOSPD2 translocated from the endoplasmic r

SUBMITTER: Zhu TF 

PROVIDER: S-EPMC12780491 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature