Single-cell atlas of grass carp ( <i>Ctenopharyngodon idella</i>) peripheral blood IgM ⁺ B cells provides insights into B cell-mediated immune responses in teleost fish.

ABSTRACT: Teleost peripheral blood contains a remarkably high proportion of B cells, accounting for 15%-50% of circulating lymphocytes. However, their immune responses to bacterial infection are yet to be elucidated. In the present study, 10× Genomics single-cell RNA sequencing (scRNA-seq) was employed to characterize the transcriptomic landscape of peripheral blood IgM ⁺ B cells in grass carp ( Ctenopharyngodon idella) following challenge with Aeromonas hydrophila, a major aquatic pathogen. Six transcriptionally distinct IgM ⁺ B cell subpopulations were identified, including (im)mature B cells, innate B cells, proliferating B cells, IgD ^high B cells, and two infection-induced subsets denoted as infection Ⅰ and Ⅱ B cells. Bacterial infection altered the

SUBMITTER: Pan YR 

PROVIDER: S-EPMC12780497 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature