Two Molecular Subgroups Predict Most Recurrences in Advanced Laryngeal Squamous Cell Carcinoma.

ABSTRACT: The aim of this study was to test whether intratumoral heterogeneity explains inconsistent reports on VEGF-A as a prognostic marker in laryngeal squamous cell carcinoma (LSCC) and to identify expression phenotypes (mRNA/miRNA) associated with recurrence. In a prospective cohort of 60 patients with T3/T4 LSCC undergoing primary laryngectomy, we sampled four regions per case (tumor surface, tumor depth, peritumoral mucosa ≤1 cm, and paired distant normal mucosa). mRNA levels of hypoxia-inducible factor (HIF) 1α/2α/3α, VEGF-A, VEGFR1/2, and ETS-1 were quantified by qRT-PCR; cohort-matched miRNA profiling (microarray with qRT-PCR validation) was integrated. Elevated VEGF-A at tumor depth predicted recurrence (log-rank P = 0.0001), whereas surface VEGF-A had no prognostic value (P = 0.170). Pai

SUBMITTER: Popov TM 

PROVIDER: S-EPMC12780839 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature