Therapy-induced remodeling of IgG and plasma N-glycans in breast cancer.

ABSTRACT:

Background

Protein N-glycosylation plays a key role in cancer biology and may offer insights into tumor behavior and treatment response. This study investigated changes in N-glycosylation of immunoglobulin G (IgG) and total plasma proteins in patients with breast cancer undergoing neoadjuvant chemotherapy.

Methods

A prospective cohort of 34 women with early-stage or locally advanced breast cancer was recruited. Plasma samples were collected before and after neoadjuvant chemotherapy. IgG was isolated by immunoaffinity chromatography, and IgG and total plasma N-glycans were enzymatically released, fluorescently labeled, and analyzed using ultra-high performance liquid chromatography. Glycan traits were expressed as relative abundances and summarized into derived structural fea

SUBMITTER: Maricic Vrban M 

PROVIDER: S-EPMC12781513 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature