LncRNA FEZF1-AS1 regulates the gene expression and alternative splicing associated with osteosarcoma.

ABSTRACT:

Background

Osteosarcoma (OS) faces a high metastatic burden, limited effective treatments, and a lack of specific therapeutic targets.

Methods

In this prospective study, we established a human osteosarcoma cell (HOS) model overexpressing lncRNA FEZF1-AS1 (OE FEZF1-AS1), and subsequently obtained FEZF1-AS1-affected transcriptome data using high-throughput RNA sequencing (RNA-seq).

Results

OE FEZF1-AS1 promoted HOS cell proliferation and invasion, upregulated 125 genes, suppressed 48 genes, and impacted 1156 alternative splicing events (ASEs), including pathways like C-type lectin receptor signaling and Yersinia pestis infection.

Conclusions

LncRNA FEZF1-AS1 plays a pivotal role in promoting the proliferation and invasion of HOS cells. Moreover, our study provide

SUBMITTER: Han Y 

PROVIDER: S-EPMC12781780 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature