RNA polymerase II degradation triggered by DNA repair occurs in trans and independently of how the lesion is recognized.

ABSTRACT: In response to DNA damage, RPB1, the catalytic subunit of RNA Polymerase II (RNAPII), is degraded by the ubiquitin-proteasome system. Degradation models only consider transcriptionally engaged molecules, where a stalled RNAPII complex functions as a lesion-recognition factor, and its RPB1 subunit is proposed to be subsequently degraded to facilitate access of lesion-processing nucleotide excision repair (NER) factors. This transcription-coupled repair is complemented by the global genome repair (GG-NER) system, where lesions are recognized by the XPC and DDB2 factors. Here, we show that RPB1 degradation is controlled in trans by a pathway that depends on lesion processing by NER, irrespectively of whether the lesion is recognized by RNAPII itself or by XPC-DDB2. Incomplete repair due to ab

SUBMITTER: Choudhary R 

PROVIDER: S-EPMC12781871 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature