YAP-TEAD regulates the super-enhancer network to control early surface ectoderm commitment.

ABSTRACT: Super enhancers (SEs), characterized by clusters of enhancers, are instrumental in shaping cellular identity and function. Given this crucial involvement of SEs in cell lineage commitment, and considering the pivotal position of surface ectoderm in differentiating into a wide array of cell types, the study of these SEs holds immense promise for advancing cell-based therapeutic applications. In this study, we profiled the SE landscape in surface ectoderm cells derived from pluripotent stem cell differentiation. By leveraging 3D genomic data, we discerned active histone modifications and frequent chromatin interactions of SEs with target genes. Notably, perturbing specific SE using a CRISPR-dCas9-mediated approach resulted in decreased expression of the connected gene. Subsequently, we const

SUBMITTER: Wang Z 

PROVIDER: S-EPMC12781878 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature