Bioproduction of <i>N</i> ⁶-benzyladenine <i>N</i> ⁹-β-D-glucopyranoside using suspension cells of bamboo (<i>Phyllostachys nigra</i>).

ABSTRACT: Suspension-cultured cells of a temperate bamboo species (Phyllostachys nigra) accumulate substantial amounts of hydroxycinnamic acid derivatives and lignin under culture conditions that promote xylogenesis. In our previous study, we found a metabolite specifically produced in bamboo cells cultured under lignification-inducing conditions in a medium containing N ⁶-benzyladenine (BA), but the chemical structure was not elucidated. In this study, we purified and identified this compound as BA N ⁹-β-D-glucopyranoside (BA-9G). Despite the presence of three nitrogen positions (N-3, N-7, and N-9) that may be glucosylated in the adenine moiety of BA, bamboo cells specifically produced BA-9G (i.e., without other glucoside types) when c

SUBMITTER: Nomura T 

PROVIDER: S-EPMC12781912 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature