Renalase stimulates aldosterone production via PMCA4b/cAMP in NCI-H295R cells.

ABSTRACT: We recently observed a significantly higher level of renalase (RNLS) in aldosterone-producing adenomas (APAs) than in the APA-adjacent adrenal glands (AAGs). RNLS is a flavin adenine dinucleotide-dependent monoamine oxidase. In this study, we investi-gated the effect of RNLS on adrenocortical aldosterone production. RNLS upregulated aldosterone production in adrenocortical cells without interfering with cell proliferation. RNLS (4 μg/ml) increased the mRNA expression of HSD3B2 (p = 0.0128) and CYP21A2 (p = 0.0013) and markedly stimulated that of CYP11B2 (p < 0.0001). Regarding the mechanism, we excluded classical calcium signalling stimulation and found that RNLS activated cAMP/PKA signalling and then upregulated the transcription factor NR4A2 and the phosphorylation o

SUBMITTER: Fu R 

PROVIDER: S-EPMC12781950 | biostudies-literature | 2026 Dec

REPOSITORIES: biostudies-literature