Cholesterol and steroid synthesis pathways may be involved in the inhibition of osteosarcoma cell viability by calcium-sensing receptor antagonism.

ABSTRACT:

Background

This study examined the effect of calcium-sensing receptor (CaSR) antagonism on human osteosarcoma cells and investigated the underlying molecular mechanisms of this effect through transcriptome sequencing.

Methods

Human osteosarcoma cell lines MG-63 and Saos-2 were treated with different concentrations (0.1-10 µM) of the CaSR antagonist NPS-2143. Cell Counting Kit-8 (CCK-8) assays were used to detect the effect of CaSR antagonism on the viability of the cells. RNA sequencing was performed on cells treated with five µM NPS-2143 for 24 hours, followed by bioinformatic analysis to identify differentially expressed genes and enriched pathways. qRT-PCR was conducted to validate key genes.

Results

CCK-8 assays showed that at low concentrations (0.1 and one µM),

SUBMITTER: Wang L 

PROVIDER: S-EPMC12782028 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature