Apicobasal Polarity Protein PARD3 Plays an Essential Role in Human Corneal Epithelial Cells.

ABSTRACT:

Purpose

Previously, we reported that the apicobasal polarity-determinant Pard3 regulates mouse corneal epithelial (CE) stratification and barrier function. Herein, we describe a complementary cell culture model to study the functions of PARD3 in greater detail.

Methods

PARD3-knockout (PARD3KO) cells were generated by CRISPR/Cas9-mutagenesis in hTCEpi, a telomerase-immortalized human CE cell line. We compared hTCEpi and PARD3KO cellular proliferation by CellTiter-Blue, crystal violet, xCELLigence Real Time Cell Analyzer, Ki67 staining and flow cytometry, extracellular matrix (ECM) affinity by adhesion assays and trypsinization time, actin polymerization by phalloidin staining, barrier function by Real-Time Cell Analyzer and FITC-dextran permeability assays, and relevant marke

SUBMITTER: Kumar S 

PROVIDER: S-EPMC12782202 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature