Genomic and chemical insights into a human lectin-binding extracellular polysaccharides from Parageobacillus toebii strain H-70.

ABSTRACT: Extracellular polysaccharides (EPSs) from thermophilic bacteria are promising biopolymers due to their stability and structural variability. This study aimed to characterize the genomic and chemical features of EPS produced by Parageobacillus toebii strain H-70 isolated from a geothermal spring in Armenia. EPS from strain H-70 was produced in sucrose and glucose based medium and analyzed chemically by TLC, HPAEC-PAD, GC-MS, and NMR. Protein, uronic acid, and nucleic acid contents were quantified by spectrophotometric methods. Molasses, an inexpensive byproduct of sugar production, was used as the carbon source too. Whole-genome sequencing, comparative phylogenomics, and genome mining were performed to identify biosynthetic gene clusters, carbohydrate-active enzymes (CAZymes), and regulator

SUBMITTER: Ghevondyan D 

PROVIDER: S-EPMC12782446 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature