Application of the solvent effect on bioluminescent reporter bacteria as a real-time membrane toxicity assay.

ABSTRACT: Bioluminescent bioreporters are widely used across various scientific disciplines due to the well-characterized bacterial bioluminescence mechanism. However, solvent-induced membrane perturbations may confound the use of bioreporters in assessing cellular toxicity from environmental contaminants. This study investigated the solvent effect, wherein membrane damage increases intracellular availability of bioluminescent reaction precursors, increasing the light produced. A new online in situ monitoring system was also tested with multiple bioluminescent reporters, including a newly constructed Pseudomonas fluorescens M3A strain, exposed to toluene, trichloroethylene, acetone, phenol and creosote derived from beechwood tar. Additional tests included the introduction of carbon nan

SUBMITTER: Myer PR 

PROVIDER: S-EPMC12782483 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature