Mitochondrial Genome Characterization of Six Spiny Crawler Mayflies and Comparative Analysis Within Ephemerellidae (Ephemeroptera: Pannota).

ABSTRACT: Mitogenomes provide important molecular information for resolving evolutionary relationships in mayflies. However, both the evolutionary patterns of mitogenomes and the phylogenetic structure of Ephemerellidae remain insufficiently understood. In this study, we sequenced six complete mitogenomes representing five ephemerellid genera and performed comprehensive comparative analyses that included all currently available ephemerellid mitogenomes. Our results reveal that trnI-associated inversion and translocation are characteristic features of Ephemerellidae mitogenomes, likely generated through tandem duplication followed by random loss during mitochondrial evolution. All species exhibited strong A + T bias and distinct compositional asymmetry, with codon usage heavily skewed toward A

SUBMITTER: Xue C 

PROVIDER: S-EPMC12782776 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature