Fibre orientations in collagen-containing tissues revealed with computational scattered light imaging and polarimetric second harmonic generation microscopy.

ABSTRACT: Collagen forms dense fibre networks in the human body, with the organisation directly influencing tissue mechanics and function in health and disease. A good understanding of this relation requires proper imaging techniques for visualising the dense collagen network. Previously, computational scattered light imaging was employed as a fast and easy-to-implement technique to retrieve the orientations of multi-directional fibres in various tissue samples, but the fibre orientations were not yet validated quantitatively in regions containing collagen fibres. In this study, we validate the in-plane orientations of fibres in collagen-containing tissues (rat tendon and bone sections) determined with computational scattered light imaging by performing comparative measurements with polarimetric sec

SUBMITTER: Ettema L 

PROVIDER: S-EPMC12783134 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature