Development and validation of a multiclass LC-MS/MS method for the analysis of cyanotoxins.

ABSTRACT: Cyanobacteria are prokaryotic organisms that can form large monospecific blooms, which pose a risk to human and animal health as some species produce toxic secondary metabolites called cyanotoxins. Multiclass cyanotoxin analysis is challenging due to varying chemical and physical properties between classes, as well as potentially large numbers of analogues within each class. Incorporating anatoxins (ATXs) into multiclass methods can be particularly challenging due to their small molecular size, potential interferences, polarity, and a lack of chemical standards for most analogues. Here, we present the development of a multiclass LC-MS/MS method and a quantitative calibration solution for aetokthonotoxin (AETX), an emerging cyanotoxin linked to mass mortalities of bald eagles in the Eastern

SUBMITTER: Zamlynny L 

PROVIDER: S-EPMC12783199 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature