BMSC-Derived Exosomal miR-21a-5p Ameliorates Blood-Brain Barrier Injury and Hemorrhagic Transformation.

ABSTRACT: Hemorrhagic transformation (HT) and disruption of the blood-brain barrier (BBB) injury are critical pathological features of ischemic stroke, profoundly influencing the natural course of the disease and treatment outcome. This study aims to elucidate how bone marrow stromal cell-derived exosome (BMSC-exo) influence BBB injury and HT and the related molecular mechanisms, focusing on the core function of exosome miR-21a-5p. The HT model induced by middle cerebral artery occlusion (MCAO-HT) was treated with tail vein injection of BMSCs-exo, and HT, BBB injury and brain damage were assessed by mNSS score, triphenyltetrazolium chloride (TTC) staining, evan's blue (EB)/hemoglobin leakage assay, and HE staining. High abundance miRNAs in BMSC-exo were identified by Small RNA sequence. BMSC-exo miR

SUBMITTER: Yang J 

PROVIDER: S-EPMC12783216 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature