Fluorescence Mean-Lifetimes of a Series of Small and Bright Fluorescent Dyes.

ABSTRACT: Fluorescence mean-lifetime is a photophysical parameter that is used in basic research and in many fields of applications. However, the parameter is not available in the literature for some small and bright fluorescent dyes, or at least it is difficult to find. Therefore, in the present work, the fluorescence mean-lifetime of some small and bright fluorescent dyes was measured in aqueous buffered solutions. A few more dyes with an already known fluorescence mean-lifetime are also included for comparison. The total list of five classes (scaffolds) and their respective dyes analyzed are as follows: (1) four rhodamines-5(6)-carboxy-X-rhodamine (ROX), Rho 6G 3⁺ (N-[2-(2-aminoethylamino)ethyl]rhodime 6G-amide), Rho 6G 2⁺ (N-(8-amino-3,6-dioxaoctyl)rhodamine 6G-amide), and

SUBMITTER: Boni L 

PROVIDER: S-EPMC12783449 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature