Integration of multi-modal monitoring for dynamic control of large-scale 3D tissue bioreactors.

ABSTRACT: The translation of tissue engineering toward clinically relevant large-scale biofabrication requires continuous and non-invasive monitoring of tissue maturation. However, few studies provide an integrated and operational demonstration of how such tracking can be effectively achieved in real bioreactor environments. Here, we propose and experimentally validate a modular analytical framework that integrates physicochemical, metabolic, morphological, and perfusion monitoring strategies designed for centimeter-scale engineered tissues cultivated under perfusion. A custom perfusion bioreactor system was developed for the cultivation of 10 cm³ bioprinted fibroblast tissues, featuring real-time online monitoring of the physicochemical environment-i.e., temperature, pH, and O₂

SUBMITTER: Chastagnier L 

PROVIDER: S-EPMC12783805 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature