Pioneer Factor FOXA1 Boosts CHO Cell Productivity.

ABSTRACT: Industrial production of biologics commonly involves the integration of transgenes into the genomes of host cells, such as Chinese hamster ovary (CHO) cells. A major determinant of productivity is the epigenetic control of the transgene promoter, accessibility of which can decrease during production due to the spread of heterochromatin. Pioneer factors such as forkhead box A1 (FOXA1) can bind heterochromatin, increase its accessibility and facilitate transcription of target genes. We show that FOXA1 can bind the EF1α and CMV promoters, which are widely used in industry. Overexpressing FOXA1 in CHO-K1 or an industrially-relevant CHO-DG44 cell line raised production of monoclonal antibody encoded by transgenes transcribed from these promoters. Mechanistically, this response can be attributed

SUBMITTER: Butterfield SP 

PROVIDER: S-EPMC12783867 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature