Proliferation, Adhesion, and Morphology of Bone-Derived Stromal Cells on Xenogenic Collagen Matrices: An In Vitro Study.

ABSTRACT:

Objectives

Acellular xenogeneic matrices are becoming increasingly popular for periodontal and peri-implant soft tissue augmentation and recession coverage. This study aimed to investigate the behavior of bone-derived stromal cells cultured onto three commercially available collagen membranes (Fibro-Gide®, mucoderm®, and NovoMatrix®).

Methods

Cells were isolated from alveolar bone chips of four adult healthy patients and were separately seeded on the membranes. Cells cultured in the absence of biomaterials were used as a control. Number and viability of cells on and around the membranes were evaluated at different timepoints. Actin cytoskeleton change was visualized with phalloidin staining. Markers for adhesion (VCAM-1, FAK, and fibronectin) were assessed by immunofluoresce

SUBMITTER: Brunello G 

PROVIDER: S-EPMC12784116 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature