SPAP: Soluble Human Plasma Proteoform Analysis via Acetonitrile Precipitation and Top-Down Mass Spectrometry.

ABSTRACT: Advances in liquid chromatography-mass spectrometry have significantly improved proteomic analyses of human plasma. However, information at the level of intact proteoforms remains limited due to the high dynamic range of protein abundance and the complexity of post-translational modifications. To address this challenge, we introduce soluble plasma proteoform analysis via acetonitrile precipitation (SPAP), a streamlined workflow for top-down mass spectrometry-based proteomics that isolates small, intact proteoforms from the acetonitrile-soluble plasma fraction, enabling direct measurement of proteoform diversity and post-translational modifications with high resolution. This simple and scalable method employs cold acetonitrile to precipitate abundant plasma proteins, thereby enriching the s

SUBMITTER: Sanchez A 

PROVIDER: S-EPMC12784391 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature