HydroBot: Software for Interactive Hydrogen/Deuterium-Exchange Mass Spectrometry Multistate Analysis.

ABSTRACT: Hydrogen/deuterium-exchange mass spectrometry (HDX-MS) is a powerful technique for probing protein dynamics, stability, and interactions. However, multistate and nonequilibrium experiments currently do not have available analysis tools. We present HydroBot, a software designed for comprehensive and interactive HDX-MS data analysis and visualization. HydroBot supports rapid and automated uptake plotting, statistical testing of differences between protein states, and multiple interactive visualization modes including bar plots, Woods plots, and heatmaps. Statistical tools such as volcano plots and error distribution analyses are integrated to assess data robustness, enabling interactive exploration of labeling differences. Correlated structural dynamics are revealed by k-means or hier

SUBMITTER: Kish M 

PROVIDER: S-EPMC12784399 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature