Physicochemical features of intrinsically disordered regions predict DNA-demethylation-promoting activity of transcription factors.

ABSTRACT:

Background

Transcription factor (TF)-dependent DNA demethylation is associated with generation of specific DNA methylation profiles in normal cellular development and disease, although only a small fraction of TFs are known to promote DNA demethylation.

Results

Here, we systematically predicted which TFs have DNA-demethylation-promoting activity. Experiments with deletion mutants of the TFs RUNX1 and SPI1 revealed that this activity is associated with a relatively long intrinsically disordered region (IDR). Examination of the IDRs from eight TFs previously confirmed to have such activity revealed that at least one IDR was active in each TF. We constructed a Random Forest classifier based on 25 numeric physicochemical features extracted from length-controlled 26 positive (act

SUBMITTER: Qin XY 

PROVIDER: S-EPMC12784575 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature