Generation of Novel High-Quality Small-Grained Rice Germplasm by Targeting the <i>OsVIN2</i> Gene.

ABSTRACT: Small-grained rice varieties are highly valued in hybrid seed production and food markets because of their unique advantages in mechanized seed production and cooking qualities. Developing new varieties combining small grain size with high nutritional and cooking quality represents an important breeding objective. The OsVIN2 gene has been identified as a key regulator involved in carbohydrate metabolism and grain development in rice. In this study, a CRISPR/Cas9-mediated gene-editing approach was employed to precisely modify the OsVIN2 gene in the restorer line MH86 of high-quality rice varieties. Compared with the wild-type plants, the frameshift mutant seeds showed a significant reduction in length and width by 19.9% and 15.2%, respectively, leading to a 39.2% decrease in t

SUBMITTER: Chen X 

PROVIDER: S-EPMC12784667 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature