Establishment of a CRISPR/Cas9-Based Genome Editing System in <i>Macrobrachium rosenbergii</i>.

ABSTRACT: The giant freshwater prawn Macrobrachium rosenbergii is a globally farmed decapod species of high economic and nutritional value, but its genetic improvement has been constrained by the lack of an efficient genome editing workflow. Here, we optimized a CRISPR/Cas9 microinjection system for targeted mutagenesis in M. rosenbergii embryos. A controlled mating-based approach enabled routine collection of one-cell embryos, and developmental staging identified 0.5-2 h post-fertilization as the optimal injection window. Optimization of the needle tip (~1 μm) minimized mechanical damage, lowering early embryo mortality to ~10%. Using this system, the eye-development gene MrPAX6 and the sex-related gene MrIAG were successfully edited. MrPAX6 editing produced a 46.

SUBMITTER: Yan J 

PROVIDER: S-EPMC12784933 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature