Phenotypic Profiling and Activation-Associated Expression of CD99 Ligands on Human Leukocytes.

ABSTRACT: The immune system comprises a complex network of cells that continuously change during activation, infection, and the maintenance of balance. Immunophenotyping offers valuable insights into the regulation of immune responses. We systematically characterized the expression profile of CD99 ligands across distinct immune cell subsets using both conventional and high-dimensional flow cytometry. CD99 ligands were detected on NK cells and monocytes under both resting and IL-2-activated conditions, with non-classical monocytes and CD56 Dim NK cells exhibiting the highest expression levels. Notably, ligand expression in these subsets was further enhanced following IL-2 activation. In contrast, T lymphocytes (CD3⁺) displayed low basal levels of CD99 ligand expression, which increased mod

SUBMITTER: Thu MM 

PROVIDER: S-EPMC12785092 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature