Overexpression of <i>GmCSY3</i> Enhances Soybean Tolerance to Excess Iron and Aluminum.

ABSTRACT:

Background

Plant citrate synthase (CSY) is involved in the iron deficiency (-Fe) response and aluminum (Al) detoxification. However, knowledge of CSY function in responding to excess iron (+Fe) or Al stress (+Al) is still limited.

Methods

The CDS and promoter of GmCSY3 were isolated from soybean and bioinformatically analyzed. The GmCSY3 expression was detected by qRT-PCR and GUS assay. The growth of GmCSY3 recombinant yeast under +Fe or +Al was detected. The phenotype, CSY activity, citric acid concentration, chlorophyll content, MDA, H₂O₂, O₂^- contents, GST, CAT, SOD, and POD activities were examined in GmCSY3 overexpressed and RNAi-suppressed soybean chimeras under +Fe or +Al. Perls and Hematoxylin st

SUBMITTER: Liu Z 

PROVIDER: S-EPMC12785149 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature