Identification of miR171a-<i>GRAS50</i> Regulatory Module Associated with Wood Properties in <i>Populus tomentosa</i>.

ABSTRACT: Enhancing wood properties, particularly fiber length (FL), represents a critical objective in Populus tomentosa breeding programs. However, the molecular mechanisms regulating these traits remain largely elusive. Here, an integrative analysis of the PtomiR171 family, uncovering substantial functional divergence among PtomiR171 family members and identified a PtomiR171a-PtoGRAS50 regulatory axis that may control cellulose-related gene expression and influence fiber development in P. tomentosa. Single-nucleotide polymorphism (SNP)-based association studies implicated the role of the PtomiR171a-PtoGRAS50 module in modulating FL. Combined with dual-luciferase reporter gene assay, real-time reverse transcription polymerase chain reaction (RT-qPCR), transcriptome and

SUBMITTER: Shi G 

PROVIDER: S-EPMC12785576 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature