A Visual and Rapid PCR Test Strip Method for the Authentication of Sika Deer Meat (<i>Cervus nippon</i>).

ABSTRACT: The rising price of sika deer meat is increasing the risk of economic adulteration, highlighting the need for rapid and reliable authentication methods to protect both market integrity and consumers. This work presents a novel countermeasure: a polymerase chain reaction (PCR)-based nucleic acid test strip designed for the specific and visual identification of sika deer meat. Our approach commenced with the design of specific primers targeting the cytochrome C oxidase subunit I (COI) gene. To guarantee the reliability of the assay, a DNA standard plasmid was constructed to serve as an unambiguous positive control for the PCR. Under optimized conditions, results showed that authentic sika deer meat generated both test and control lines on the strip, while adulterated and negative samp

SUBMITTER: Gao L 

PROVIDER: S-EPMC12785597 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature