Advancing the Analysis of Fatty Acid Composition in Animal-Based Marine Oils Through the Integration of Raman and IR Spectroscopy with Chemometrics.

ABSTRACT: This study investigated the use of Raman and IR spectroscopy, individually and combined, for quantifying fatty acid methyl ester (FAME) profiles in animal-based marine oils and potential adulterants (palm oil, ω-3 concentrates in ethyl ester, and generic fish oil). FAME profiles are important for assessing oil quality, conventionally determined via gas chromatography. This study aimed to provide a rapid and non-destructive alternative. The study utilized Partial Least Squares Regression (PLSR) alongside Raman (r² = 0.94; RMSEP = 2.4%) and IR spectroscopy (r² = 0.95; RMSEP = 2.3%), demonstrating similar ω-3 fatty acid predictions. Fusion of IR and Raman spectroscopic datasets improved ω-3 fatty acid (r² = 0.96; RMSEP = 1.9%), polyunsaturated fatty acids (PUF

SUBMITTER: Ahmmed F 

PROVIDER: S-EPMC12785698 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature