Phosphoproteomic Profiling Reveals Overlapping and Distinct Signaling Pathways in <i>Dictyostelium discoideum</i> in Response to Two Different Chemorepellents.

ABSTRACT: Chemorepulsion mechanisms for eukaryotic cells are poorly understood. We performed proteomics and phosphoproteomics to elucidate how Dictyostelium discoideum responds to its two endogenous chemorepellent signals, the protein AprA and inorganic polyphosphate (polyP). AprA and polyP affected levels of more than 200 proteins, with an overlap of both upregulating 25 proteins and downregulating two proteins. Two proteins were upregulated by AprA but downregulated by polyP, while two others showed the opposite trend. Surprisingly, many of the AprA- and polyP-regulated proteins are associated with RNA metabolism and ribosomes. AprA increased phosphorylation of 15 proteins and decreased phosphorylation of 36 proteins. PolyP increased phosphorylation of 12 proteins and decreased phosphorylat

SUBMITTER: Uddin SZ 

PROVIDER: S-EPMC12785797 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature