Metabolomic Profiling of Middle Ear Effusion Suggests a Predominant Influence of Age over Viscosity: An HR-MAS NMR Study.

ABSTRACT: Otitis media with effusion (OME) involves heterogeneous middle ear effusion (MEE), and its classification based on viscosity (serous/mucous) is often confounded by patient age. This study determined the independent contributions of age and viscosity to the MEE metabolome. In this prospective study, high-resolution magic-angle spinning nuclear magnetic resonance analysis of 83 MEE samples (45 adult serous, 17 child serous < 12 years, and 21 child mucous) was performed. Statistical analyses included principal component analysis, correlation analysis, analysis of covariance (ANCOVA), and age-adjusted receiver operating characteristic curve analysis. Age was the primary metabolic variance determinant, overshadowing viscosity-related differences. Significant age-associated metabolic trends were

SUBMITTER: Lee S 

PROVIDER: S-EPMC12785847 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature